Degree Type

Thesis

Date of Award

2008

Degree Name

Master of Science

Department

Chemical and Biological Engineering

First Advisor

Balaji Narasimhan

Second Advisor

Michael Wannemuehler

Third Advisor

Aaron Clapp

Abstract

The objective was to study the chemistry effect on protein release and stabilization and on immune cell activation using polyanhydrides. The two goals were to: (1) Determine how polyanhydride chemistry and fabrication methods affect the release kinetics of proteins from microspheres and the stability of the released protein. Microspheres were fabricated using two non-aqueous methods: solid/oil/oil and cryogenic atomization. Studies found no significant difference in release kinetics of ovalbumin. The more hydrophilic polyanhydrides showed more favorable protein stability, preserving both the immunological epitopes and the primary structure. (2) Investigate the surface marker expression of CD86, CD40, DC SIGN, and MHCII on dendritic cells by polyanhydride microspheres and study the effect of chemistry on activation pathways of these immune cells. All microspheres were shown to induce maturation of murine dendritic cells; the degree to which each surface marker tested was activated was a function of chemistry.

DOI

https://doi.org/10.31274/rtd-180813-16050

Publisher

Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/

Copyright Owner

Senja Katarina Lopac

Language

en

Proquest ID

AAI1450148

OCLC Number

229406120

ISBN

9780549422280

File Format

application/pdf

File Size

102 pages

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