Date of Award
Master of Science
Chemical and Biological Engineering
The objective was to study the chemistry effect on protein release and stabilization and on immune cell activation using polyanhydrides. The two goals were to: (1) Determine how polyanhydride chemistry and fabrication methods affect the release kinetics of proteins from microspheres and the stability of the released protein. Microspheres were fabricated using two non-aqueous methods: solid/oil/oil and cryogenic atomization. Studies found no significant difference in release kinetics of ovalbumin. The more hydrophilic polyanhydrides showed more favorable protein stability, preserving both the immunological epitopes and the primary structure. (2) Investigate the surface marker expression of CD86, CD40, DC SIGN, and MHCII on dendritic cells by polyanhydride microspheres and study the effect of chemistry on activation pathways of these immune cells. All microspheres were shown to induce maturation of murine dendritic cells; the degree to which each surface marker tested was activated was a function of chemistry.
Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/
Senja Katarina Lopac
Lopac, Senja Katarina, "Polymer chemistry effects on protein release and immune activation" (2008). Retrospective Theses and Dissertations. 14904.