Degree Type

Thesis

Date of Award

2008

Degree Name

Master of Science

Department

Theses & dissertations (Interdisciplinary)

Major

Food Science and Human Nutrition

First Advisor

Manju B. Reddy

Second Advisor

Kevin Schalinske

Third Advisor

M. Paul Scott

Abstract

Biofortification is a strategy for combating iron deficiency that involves the selective breeding of food crops to increase total bioavailable iron. Evidence that ferritin is a good source of bioavailable iron has created interest for increasing ferritin concentrations in seeds. However the successful implementation of ferritin biofortification requires a method to rapidly measure ferritin in staple food crops. In this study, we developed an indirect ELISA to quantify ferritin in red beans, white beans, wheat, maize, and brown rice. This required the production of an anti-ferritin antibody that is immunoreactive with all seed ferritins, the development of a rapid method for the crude extraction of ferritin from the seeds, and the use of immunoblotting techniques to verify immunoreactivity of the antibodies. This simple and reliable assay will facilitate the rapid screening of large numbers of seeds that can be used by plant breeders to identify and breed ferritin-rich crops.

DOI

https://doi.org/10.31274/rtd-180813-16061

Publisher

Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/

Copyright Owner

Rebecca Jane Lukac

Language

en

Proquest ID

AAI1453050

OCLC Number

235943413

ISBN

9780549540687

File Format

application/pdf

File Size

83 pages

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