Degree Type

Thesis

Date of Award

2007

Degree Name

Master of Science

Department

Biochemistry, Biophysics and Molecular Biology

First Advisor

Alan Myers

Second Advisor

Martha James

Abstract

The roles of three starch branching enzyme isoforms, BE1, BE2 and BE3, in Arabidopsis leaf starch metabolism were investigated by AtBE mutant analysis. We also determined the expression patterns of BEs in Arabidopsis by GUS staining and microarray experiments. The possible correlation of BE gene expression with other genes in the Arabidopsis genome was analyzed to identify putative genes that are functionally correlated with BEs. Motifs in the promoters of the Arabidopsis BE genes were identified to better understand their expression differences. BE2 and BE3 expression levels during a diurnal cycle were evaluated with isoform specific antibodies. Meantime, the in vitro biochemical activities of purified recombinant BE2 and BE3 proteins were investigated by three different assays to better understand their specific functions in starch biosynthesis. Analyses of the structures of the final products from the in vitro BE-amylose reactions were done by FACE with two different forms of amylose.

DOI

https://doi.org/10.31274/rtd-180813-16179

Publisher

Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/

Copyright Owner

Qinglei Gan

Language

en

Proquest ID

AAI1446041

OCLC Number

226390655

ISBN

9780549141600

File Format

application/pdf

File Size

119 pages

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