Date of Award
Master of Science
Theses & dissertations (Interdisciplinary)
Joel R. Coats
Jeffrey D. Wolt
The environmental fate and effects of a transgenic maize-expressed recombinant antigen, Escherichia coli heat-labile enterotoxin subunit B (LTB), was studied under laboratory conditions. The time for 50% dissipation (DT50) for the extractable fraction of maize-expressed LTB in soil was 35∼90 days, and both extractability and persistence were strongly affected by the soil types and environmental conditions. In addition to the environmental fate study, laboratory bioassays were conducted to investigate the sub-acute effects of maize-expressed LTB on two soil invertebrates. Both the 28-day springtail, Folsomia candida reproduction assay and earthworm, Eisenia fetida growth assay showed no adverse effects of LTB under a concentration which was well above the maximum estimated environmental concentrations. For these observations, it is predicted that the adverse effects of LTB expressing maize on F. candida and E. fetida are negligible.
Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/
Kosaki, Hirofumi, "Environmental studies on the fate and effects of pharma proteins produced in transgenic maize" (2007). Retrospective Theses and Dissertations. 15080.