Degree Type

Thesis

Date of Award

2007

Degree Name

Master of Science

Department

Theses & dissertations (Interdisciplinary)

Major

Toxicology

First Advisor

Joel R. Coats

Second Advisor

Jeffrey D. Wolt

Abstract

The environmental fate and effects of a transgenic maize-expressed recombinant antigen, Escherichia coli heat-labile enterotoxin subunit B (LTB), was studied under laboratory conditions. The time for 50% dissipation (DT50) for the extractable fraction of maize-expressed LTB in soil was 35∼90 days, and both extractability and persistence were strongly affected by the soil types and environmental conditions. In addition to the environmental fate study, laboratory bioassays were conducted to investigate the sub-acute effects of maize-expressed LTB on two soil invertebrates. Both the 28-day springtail, Folsomia candida reproduction assay and earthworm, Eisenia fetida growth assay showed no adverse effects of LTB under a concentration which was well above the maximum estimated environmental concentrations. For these observations, it is predicted that the adverse effects of LTB expressing maize on F. candida and E. fetida are negligible.

DOI

https://doi.org/10.31274/rtd-180813-16214

Publisher

Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/

Copyright Owner

Hirofumi Kosaki

Language

en

Proquest ID

AAI1446116

OCLC Number

183190519

ISBN

9780549148708

File Format

application/pdf

File Size

89 pages

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