Degree Type

Dissertation

Date of Award

2006

Degree Name

Doctor of Philosophy

Department

Theses & dissertations (Interdisciplinary)

Major

Bioinformatics and Computational Biology; Genetics;

First Advisor

Eve Syrkin Wurtele

Second Advisor

Xun Gu

Abstract

Combined bioinformatic approaches, using genomic and transcriptomic data, are applied to investigate the fatty acid biosynthesis pathway, at the molecular level, and in the context of the system biology of Arabidopsis. Fatty acids are essential components of all known bacterial and eukaryotic cells with critical role in cells as energy reserves and the metabolic precursors for biological membranes. The pathway for fatty acid synthesis seems to be conserved across all living systems. Acetyl-CoA carboxylase, a member of a superfamily of biotin-dependent enzymes, catalyzes the first committed step of the fatty acid biosynthesis pathway. Phylogenetic study exposed complex and intertwined evolutionary histories of this family, with multiple domain fusions and rearrangements. As revealed by meta-analysis of a wide array of Arabidopsis transcriptomic data, fatty acid biosynthesis is transcriptionally regulated, and this regulation not only extends across all pathway reactions, but also some substrate- and cofactor-producing reactions, thus defining a major transcriptionally co-regulated pathway. Meta-analysis of the transcriptome is extended to find groups of coexpressed genes (also called modules, or regulons) in the Arabidopsis genome. Major functionally-coherent gene groups were identified. These comprise development, information processing, defense, and metabolism, as well as tissue- and organelle-specific processes.

DOI

https://doi.org/10.31274/rtd-180813-11870

Publisher

Digital Repository @ Iowa State University, http://lib.dr.iastate.edu

Copyright Owner

Wieslawa Izabela Mentzen

Language

en

Proquest ID

AAI3229105

File Format

application/pdf

File Size

74 pages

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