Degree Type

Thesis

Date of Award

2008

Degree Name

Master of Science

Department

Mechanical Engineering

First Advisor

Pranav Shrotriya

Second Advisor

Marit Nilsen-Hamilton

Third Advisor

Sriram Sundararajan

Abstract

The first purpose of this study is to examine using AFM height measurements to detect changes in nucleic acid and protein interactions. This study used a thiolated adenine oligonucleotide (Poly A) and thymine DNA strand (Poly T) or 2 kDa poly(ethylene) glycol (PEG) as control, and the thrombin aptamer and thrombin for detection pair. The poly A and aptamer were printed using microcontact printing, then exposed to complementary species. There was a height change after binding to the target species, so it is feasible for height data to detect binding. The second purpose of this study is to use the force spectroscopy method to measure the interaction between the thrombin and its aptamer. The binding force of the thrombin-aptamer interaction was 17.8 pN, and there was little response from the controls. The results from this experiment show that the thrombin-aptamer pair has far-reaching applications for biosensors and nanostructures.

DOI

https://doi.org/10.31274/rtd-180813-16688

Publisher

Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/

Copyright Owner

Janice Dionne Marquardt

Language

en

Proquest ID

AAI1454659

OCLC Number

263683723

ISBN

9780549686378

File Format

application/pdf

File Size

39 pages

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