Date of Award
Master of Science
The first purpose of this study is to examine using AFM height measurements to detect changes in nucleic acid and protein interactions. This study used a thiolated adenine oligonucleotide (Poly A) and thymine DNA strand (Poly T) or 2 kDa poly(ethylene) glycol (PEG) as control, and the thrombin aptamer and thrombin for detection pair. The poly A and aptamer were printed using microcontact printing, then exposed to complementary species. There was a height change after binding to the target species, so it is feasible for height data to detect binding. The second purpose of this study is to use the force spectroscopy method to measure the interaction between the thrombin and its aptamer. The binding force of the thrombin-aptamer interaction was 17.8 pN, and there was little response from the controls. The results from this experiment show that the thrombin-aptamer pair has far-reaching applications for biosensors and nanostructures.
Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/
Janice Dionne Marquardt
Marquardt, Janice Dionne, "Force interaction characterization between thrombin and DNA aptamers" (2008). Retrospective Theses and Dissertations. 15466.