Date of Award
Doctor of Philosophy
Osteoarthritis (OA) is the 2nd leading cause of long term disability in North America affecting 40 million Americans. The work presented in this manuscript addresses clinical outcome measures used to determine treatment effect and 2 in vitro studies using tissue engineering techniques to grow cartilage suitable for in vivo use. In the first study, radiographs of the stifle joint in dogs were scored for the severity of OA, and limb function was measured by force platform gait analysis. The 2 outcome measures were analyzed using regression methods. There was no correlation of the severity of radiographic OA and limb function; therefore, radiographs should not be used as a measure of functional outcome for the treatment of OA. Additionally, treatment for OA using tissue engineered tissue is a current research paradigm. The second study in the manuscript hypothesized that tantalum trabecular metal was chondroconductive. Canine chondrocytes were cultured in vitro under dynamic conditions. Histopathologic evaluation showed matrix stained with toluidine blue and safranin O. Biochemical evaluation of the exterior portion of cartilage showed 84% type II collagen. The results reflected the chondroconductive potential of the unique metal. In order to optimize the medium used for in vitro cartilage production, a pellet culture model was used to determine the effect of Fibroblast Growth Factor 2 (FGF2) followed by Transforming Growth Factor beta1 (TGFbeta1) on matrix production. Although TGFbeta1 treated cultures were larger than the others, FGF2 treatment in monolayer decreased matrix production. Additionally, type II collagen was insufficient in all groups. Further study is needed to define the ideal culture requirements to produce cartilage from canine chondrocytes consistently with ideal matrix properties.
Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/
Wanda June Gordon-Evans
Gordon-Evans, Wanda June, "Osteoarthritis: clinical evaluation and cartilage tissue engineering" (2007). Retrospective Theses and Dissertations. 15536.