Putting greens are the most labor-intensive areas on golf courses; therefore, they often receive the highest inputs of fertilizers and pesticides which contain heavy metals such as Copper (Cu). Although Cu is an essential element for plants, there is some danger in an accumulation of Cu in the soil of golf course greens due to frequent and prolonged use of Cu-containing fungicides, organic fertilizers composed of sewage sludge and poultry manure, and from irrigation with sewage effluent water (Marschner, 1995). Improper management of golf course greens through over-application of heavy metals could become problematic because the Cu ion is toxic to plant cells (Ware, 1994).