Date of Award
Master of Science
Veterinary Microbiology and Preventive Medicine
The effects of Mycoplasma bovis on activation and cytotoxicity of bovine aortic and pulmonary microvascular endothelial cells were studied. Inoculation of bovine endothelial cells with M. bovis in vitro induced mediators such as vascular cellular adhesion molecule-1 and monocyte chemoattractant protein-1, which are specific for mononuclear cell but not for neutrophil extravasation. Differential activation of endothelial cells was also observed, with early activation of proinflammatory cytokines interlukin-1[beta] (IL-1[beta]) and IL-6 in microvascular endothelial cells, while induction of monocyte chemoattractant protein-1 and IL-8 was limited to microvascular endothelial cells. We also found that there were significant strain differences between responses to abscess-forming strain M. bovis 428E and nonabscess-forming strain DSA16. Specifically, only DSA16 was subcytotoxic to both endothelial cell types. As for their activation effects, significant strain differences were limited to responses in microvascular cells. Strain DSA16 induced significantly higher levels of mediators compared to strain 428E. Taken together, these results are consistent with the predominant perivascular mononuclear cell infiltration observed in M. bovis-infected lung lesions.
Lu, Xiaodong, "Differential gene expression and cytotoxicity of bovine aortic and pulmonary microvascular endothelial cells infected with Mycoplasma bovis" (2004). Retrospective Theses and Dissertations. 17512.