Degree Type

Thesis

Date of Award

1-1-2004

Degree Name

Master of Science

Department

Veterinary Microbiology and Preventive Medicine

Major

Veterinary Microbiology

Abstract

The effects of Mycoplasma bovis on activation and cytotoxicity of bovine aortic and pulmonary microvascular endothelial cells were studied. Inoculation of bovine endothelial cells with M. bovis in vitro induced mediators such as vascular cellular adhesion molecule-1 and monocyte chemoattractant protein-1, which are specific for mononuclear cell but not for neutrophil extravasation. Differential activation of endothelial cells was also observed, with early activation of proinflammatory cytokines interlukin-1[beta] (IL-1[beta]) and IL-6 in microvascular endothelial cells, while induction of monocyte chemoattractant protein-1 and IL-8 was limited to microvascular endothelial cells. We also found that there were significant strain differences between responses to abscess-forming strain M. bovis 428E and nonabscess-forming strain DSA16. Specifically, only DSA16 was subcytotoxic to both endothelial cell types. As for their activation effects, significant strain differences were limited to responses in microvascular cells. Strain DSA16 induced significantly higher levels of mediators compared to strain 428E. Taken together, these results are consistent with the predominant perivascular mononuclear cell infiltration observed in M. bovis-infected lung lesions.

Copyright Owner

Xiaodong Lu

Language

en

OCLC Number

56410528

File Format

application/pdf

File Size

70 pages

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