Date of Award
Master of Science
Veterinary Microbiology and Preventive Medicine
Enzyme linked immunosorbant assays (ELISAs) are commonly used for monitoring herd infection and diagnosing infection by Mycoplasma hyopneumoniae in individual pigs based on detection of antibodies to M. hyopneumoniae in swine serum. M. hyopneumoniae attaches to the ciliated respiratory epithelium and is minimally invasive, therefore, the serum antibody response following infection can be variable. This variable antibody response makes interpretation of assay results difficult, especially due to a high percentage of false negative test results. Accordingly, the relationship of ELISA results to the presence or absence of M. hyopneumoniae infection has been controversial. Although ELISAs for detection of M. hyopneumoniae antibodies are used extensively, there is little information in the scientific literature as to the performance attributes (i.e. sensitivity, specificity, positive predictive value, negative predictive value, etc.) of these tests. The overall objective of this thesis was to evaluate the performance of these tests. The assays were found to perform similarly, with the blocking ELISA slightly outperforming the indirect ELISAs. Specificity of the ELISAs was excellent, nearing 100%, while the specificities of the three assays were less than desired, making M. hyopneumoniae ELISAs useful for herd level monitoring, but less accurate for diagnosis in individual animals.
Keith Richard Erlandson
Erlandson, Keith Richard, "Analysis of enzyme linked immunsorbant assays for the detection of Mycoplasma hyopneumoniae antibodies in swine serum" (2004). Retrospective Theses and Dissertations. 17518.