Date of Award
Doctor of Philosophy
Biochemistry, Biophysics and Molecular Biology
Mark S. Hargrove
Hexacoordinate hemoglobins are found in a truly diverse array of organisms, ranging from cyanobacteria to humans. The prevalence of these proteins in nature coupled with very high sequence identity between species homologues suggests they have a role vital to life. The high sequence identity also implies that hxHb structural features, including the hexacoordination phenomenon, are critical aspects of the physiological function. A detailed understanding of the ligand binding behavior of these proteins will not only facilitate efforts towards discovering their physiological role(s), but will also help explain how hxHbs perform their function. This dissertation consists of four published papers which examine several hexacoordinate hemoglobins from a diverse array of species. The studies conducted in these papers range from gene discovery and mapping, tissue expression and protein biochemistry through kinetic and structural analysis.
Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/
James Thomas Trent, III
Trent, James Thomas III, "An examination of hexacoordinate hemoglobins using the techniques of biochemistry, biophysics and molecular biology " (2006). Retrospective Theses and Dissertations. 1896.