Degree Type

Dissertation

Date of Award

2006

Degree Name

Doctor of Philosophy

Department

Biochemistry, Biophysics and Molecular Biology

First Advisor

Mark S. Hargrove

Abstract

Hexacoordinate hemoglobins are found in a truly diverse array of organisms, ranging from cyanobacteria to humans. The prevalence of these proteins in nature coupled with very high sequence identity between species homologues suggests they have a role vital to life. The high sequence identity also implies that hxHb structural features, including the hexacoordination phenomenon, are critical aspects of the physiological function. A detailed understanding of the ligand binding behavior of these proteins will not only facilitate efforts towards discovering their physiological role(s), but will also help explain how hxHbs perform their function. This dissertation consists of four published papers which examine several hexacoordinate hemoglobins from a diverse array of species. The studies conducted in these papers range from gene discovery and mapping, tissue expression and protein biochemistry through kinetic and structural analysis.

DOI

https://doi.org/10.31274/rtd-180813-16479

Publisher

Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/

Copyright Owner

James Thomas Trent, III

Language

en

Proquest ID

AAI3243853

File Format

application/pdf

File Size

130 pages

Share

COinS