Structurally integrated photoluminescence-based lactate sensor using organic light emitting devices (OLEDs) as the light source

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2006-01-01
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Qian, Chengliang
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Abstract

Multianalyte bio(chemical) sensors are extensively researched for monitoring analytes in complex systems, such as blood serum. As a step towards developing such multianalyte sensors, we studied a novel, structurally integrated, organic light emitting device (OLED)-based sensing platform for detection of lactate. Lactate biosensors have attracted numerous research efforts, due to their wide applications in clinical diagnosis, athletic training and food industry. The OLED-based sensor is based on monitoring the oxidation reaction of lactate, which is catalyzed by the lactate oxidase (LOx) enzyme. The sensing component is based on an oxygen-sensitive dye, Platinum octaethyl porphyrin (PtOEP), whose photoluminescence (PL) lifetime [Tau] decreases as the oxygen level increases. The PtOEP dye was embedded in a thin film polystyrene (PS) matrix; the LOx was dissolved in solution or immobilized in a sol-gel matrix, [Tau] was measured as a function of the lactate concentration; as the lactate concentration increases, [Tau] increases due to increased oxygen consumption. The sensors' performance is discussed in terms of the detection sensitivity, dynamic range, and response time. A response time of ~ 32 sec was achieved when the LOx was dissolved in solution and kept in a closed cell. Steps towards development of a multianalyte sensor array using an array of individually addressable OLED pixels were also presented.

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Sun Jan 01 00:00:00 UTC 2006