Degree Type
Dissertation
Date of Award
1983
Degree Name
Doctor of Philosophy
Department
Microbiology
Major
Molecular, Cellular, and Developmental Biology
Abstract
Hybridomas were established that secreted antibodies specific for soybean mosaic virus (SMV), lettuce mosaic virus (LMV), maize dwarf mosaic virus A (MDMV-A), or maize dwarf mosaic virus B (MDMV-B). Virus-specific monoclonal antibody immobilized on protein A-Sepharose was active in binding virus particles from solution. Monoclonal antibodies against SMV were used in a double antibody sandwich radioimmunoassay (RIA). An RIA using one type of monoclonal antibody lacked sensitivity because limited antigenic sites were available. Results obtained using two monoclonal antibodies that bound to different antigenic sites were comparable to those obtained using polyclonal antibodies. Inhibition RIA assays of SMV, LMV, MDMV-A, and MDMV-B were sensitive to 25 ng when single monoclonal antibodies were used.
DOI
https://doi.org/10.31274/rtd-180813-5100
Publisher
Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/
Copyright Owner
Edward K. Hill
Copyright Date
1983
Language
en
Proquest ID
AAI8316148
File Format
application/pdf
File Size
89 pages
Recommended Citation
Hill, Edward K., "Production and use of monoclonal antibodies against the potato virus Y group " (1983). Retrospective Theses and Dissertations. 7638.
https://lib.dr.iastate.edu/rtd/7638
