Degree Type

Dissertation

Date of Award

1983

Degree Name

Doctor of Philosophy

Department

Microbiology

Major

Molecular, Cellular, and Developmental Biology

Abstract

Hybridomas were established that secreted antibodies specific for soybean mosaic virus (SMV), lettuce mosaic virus (LMV), maize dwarf mosaic virus A (MDMV-A), or maize dwarf mosaic virus B (MDMV-B). Virus-specific monoclonal antibody immobilized on protein A-Sepharose was active in binding virus particles from solution. Monoclonal antibodies against SMV were used in a double antibody sandwich radioimmunoassay (RIA). An RIA using one type of monoclonal antibody lacked sensitivity because limited antigenic sites were available. Results obtained using two monoclonal antibodies that bound to different antigenic sites were comparable to those obtained using polyclonal antibodies. Inhibition RIA assays of SMV, LMV, MDMV-A, and MDMV-B were sensitive to 25 ng when single monoclonal antibodies were used.

DOI

https://doi.org/10.31274/rtd-180813-5100

Publisher

Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/

Copyright Owner

Edward K. Hill

Language

en

Proquest ID

AAI8316148

File Format

application/pdf

File Size

89 pages

Included in

Microbiology Commons

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