Degree Type
Dissertation
Date of Award
1984
Degree Name
Doctor of Philosophy
Department
Horticulture
Abstract
Investigations into the nature of ethylene production by Bipolaris sorokiniana and B. sorokiniana infected leaf blades of Poa pratensis were the main objectives of this research. Characteristics of the assay for 1-aminocyclopropane-1-carboxylic acid (ACC) synthase, the enzyme that controls ethylene formation in higher plants, were also studied;Protein was found to interfere with the determination of ACC. Interference was partly overcome by modifying the assay procedure;Methionine (Met) and ACC stimulated ethylene production in B. sorokiniana. ACC was less effective than Met in stimulating ethylene production. Met decreased fungal growth and sporulation;Infection of P. pratensis by B. sorokiniana resulted in necrosis of the afflicted tissue. It was speculated that the host-pathogen interaction involved the contribution of the fungal ACC synthetic pathway and the ethylene forming pathway of the host.
DOI
https://doi.org/10.31274/rtd-180813-6119
Publisher
Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/
Copyright Owner
Lloyd W. Colemann, III
Copyright Date
1984
Language
en
Proquest ID
AAI8423697
File Format
application/pdf
File Size
96 pages
Recommended Citation
Colemann, Lloyd W. III, "Determination of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase in Poa pratensis induced by mechanical or biotic factors and characteristics of the assay for ACC " (1984). Retrospective Theses and Dissertations. 7751.
https://lib.dr.iastate.edu/rtd/7751
