Degree Type


Date of Award


Degree Name

Doctor of Philosophy


Food and Nutrition


Glutathione (GSH), produced from dietary methionine and cysteine, is involved in detoxification of many foreign compounds. The effects of dietary methionine on lead and lindane in mice and rats were studied. In three separate experiments, methionine deficient (MD) diets and methionine sufficient (MS) diets were fed to the animals. In experiment I, MS mice treated with lead acetate (250 ppm pB in their drinking water) and given lindane orally (4.6 mg once a week for 9 weeks) had a greater survival rate than similar MD mice. In Experiment II, where the lead treatment lasted only 3 weeks and lindane was given only once, the lead treatment increased blood, kidney and liver GSH levels in both diet groups. Lindane decreased liver GSH most significantly in the MD mice. In Experiment III, rats were fed MD or MS diets supplemented with lead acetate at 10,000 ppm Pb and were treated once with lindane (25% of LD(,50) for rats) orally. When MS rats were pair-fed to MD rats, liver GSH levels of both groups of rats increased by the lead and lindane, but the increase was greater in the MS fed rats. The methionine status of the animals appears to be an important factor in determining the liver GSH level.



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Copyright Owner

Victoria Ann Rowe



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74 pages