Date of Award
Doctor of Philosophy
Daniel W. Armstrong
The macrocyclic glycopeptide stationary phases have been widely used in enantiomeric separations. Some unusual aspects of this class of stationary phases were investigated in this work. The reversal of elution order among members of this class of stationary phases was studied. These chiral stationary phases were not only used in enantiomeric separations, but also for separating closely related peptides. Many of the biologically important peptides were separated with mobile phases that were compatible with ESIMS detection.;A hydrophilic OH-functionalized polystyrene divinylbenzene spherical porous particle packing was used to develop a size exclusion column for microbial analysis. The theory of the close-packing of spheres is proposed to show that common bacteria should be able to perfuse in the inter particle voids of the 50 mum spherical packing. The size exclusion column coupled with polarimetric detection was used to follow the growth of three different bacteria. Coupling this separation method to an ICP magnetic sector mass spectrometer provides an effective means to study the microbial uptake of heavy metals (i.e., uranium) from their surrounding environments. Multiple metal ions can be monitored in the microorganism and in the surrounding solution. Crucial information can be provided for the remediation of radioactive waste sites by using this method. The effect of uranium on microbial growth is also discussed.
Digital Repository @ Iowa State University, http://lib.dr.iastate.edu
Zhang, Bo, "Unusual separations with macrocyclic glycopeptide chiral stationary phases and chromatographic analysis and characterization of microbes " (2004). Retrospective Theses and Dissertations. 829.