Degree Type

Dissertation

Date of Award

1982

Degree Name

Doctor of Philosophy

Department

Theses & dissertations (Interdisciplinary)

Major

Immunobiology

Abstract

The outer envelope of Treponema hyodysenteriae strain B204 was solubilized with CHAPS detergent, a zwitterionic derivative of cholic acid. At least two T. hyodysenteriae specific proteins were identified by agar gel double diffusion and SDS PAGE of immune precipitates using rabbit anti-T. hyodysenteriae serum absorbed over a Treponema innocens strain B256, a nonpathogen, affinity column;A passive immunity study indicated swine dysentery recovered sows provided protection to their piglets via the milk up until weaning. An amplified ELISA detected IgA specific antibodies to partially purified outer envelope antigens. Levels of IgA antibodies to fractions containing T. hyodysenteriae specific proteins were associated with passive protection to swine dysentery;Four groups of pigs were vaccinated intraperitoneally with preparations containing T. hyodysenteriae specific proteins. Although protection against swine dysentery was not complete, shedding of T. hyodysenteriae and onset of clinical signs in three of the groups were delayed.

DOI

https://doi.org/10.31274/rtd-180813-2108

Publisher

Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/

Copyright Owner

Loren Harold Peterson

Language

en

Proquest ID

AAI8307779

File Format

application/pdf

File Size

110 pages

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