Degree Type

Dissertation

Date of Award

1983

Degree Name

Doctor of Philosophy

Department

Zoology

Abstract

Nuclei from Drosophila melanogaster embryos contain two major nonhistone chromosomal proteins which are extracted by 0.35 M NaCl and by 2% perchloric acid. A63 (M(,r) about 63,000) and A13 (M(,r) about 10,000) both contain high levels of basic and acidic amino acids, a property characteristic of high mobility group (HMG) proteins isolated from vertebrate tissues. Polytene chromosomes were stained by immunofluorescent techniques with anti-A13 polyclonal antibodies. The staining pattern indicates that A13 does not specifically react with puffs, nor is the protein preferentially associated with heterochromatin or nucleoli;There appears to be multiple binding sites for A13 throughout nuclei. A13 is rapidly released from nuclei by micrococcal nuclease. A13 cannot be completely released from nuclei by this nuclease, however, since even at very high digestion levels, the protein is also detected associated with nucleosomal fractions. At these high digestion levels, only histone Hl-depleted mononucleosomes are solubilized in 5 mM EDTA. Some of these mononucleosomes also contain A13. The results indicate that A13 closely resembles the properties possessed by vertebrate HMGs 1 and 2.

DOI

https://doi.org/10.31274/rtd-180813-8557

Publisher

Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/

Copyright Owner

James Andrew Bassuk

Language

en

Proquest ID

AAI8407051

File Format

application/pdf

File Size

127 pages

Included in

Zoology Commons

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