Date of Award
Doctor of Philosophy
Gerald J. Small
The application of fluorescence line narrowing spectrometry (FLNS) to the investigation of polar polycyclic aromatic hydrocarbon (PAH) metabolites and their corresponding DNA adducts is demonstrated. The selectivity is shown through the successful resolution of all components in separate mixtures of similar but distinct derivatives of benzo (a) pyrene, benz (a) anthracene, and chrysene. The separate mixtures were composed of six metabolites, five DNA adducts, each metabolite and its corresponding DNA adduct, and six metabolites and two DNA adducts. A limit of detection of ~3 modified bases in 10[superscript]8 (20 [mu]g DNA) was determined using the adduct formed between the diol epoxide of benzo (a) pyrene and DNA (BPDE-DNA). In order to achieve this limit of detection, measures to avoid photooxidation and nonphotochemical hole burning (NPHB) had to be employed;The broad applicability of FLNS is demonstrated through applications to the analysis of globin adducts, PAH metabolites in urine, and real samples, and to the investigation of carcinogenic metabolic pathways. ftn[superscript] aDOE Report IS-T-1328. This work was performed under contract No. W-7405-Eng-82 with the U.S. Department of Energy.
Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/
Richard Scott Cooper
Cooper, Richard Scott, "High resolution-sensitivity charcterization of polycyclic aromatic hydrocarbon-DNA adducts using fluorescence line narrowing spectrometry " (1987). Retrospective Theses and Dissertations. 8628.