Date of Award
Doctor of Philosophy
Veterinary Microbiology and Preventive Medicine
This study involved the identification and characterization of detergent-soluble bovine herpesvirus-1 (BHV-1) specified antigens involved in inducing serum neutralizing antibody. Bovine herpesvirus-1 infected cell antigens were solubilized with Nonidet P-40. Monospecific sera against individual BHV-1 antigens were prepared by preparative line immunoelectrophoresis; individual immunoprecipitates were used to immunize rabbits;A method was devised to test the monospecificity of the rabbit sera. A BHV-1 crossed immunoelectrophoretic (CIE) pattern was formed using the crude antigen extract and bovine hyperimmune serum. The CIE pattern was transferred electrophoretically to a nitrocellulose sheet. Transferred antigen was able to react with rabbit sera (detected by ('125)I-labeled anti-species antibody); autoradiography revealed the antigen arc(s) for which the sera were specific;Monospecific rabbit sera possessing anti-BHV-1 serum neutralizing activity were analyzed by reaction with ('35)S-methionine or ('14)C-glucosamine-labeled crude antigen extract in immunoprecipitation sodium dodecylsulfate polyacrylamide gel electrophoresis and Western blot. Results indicated that a 77-81K dalton glycopeptide and an 82-92K dalton glycopeptide were involved, separately, in inducing serum neutralizing antibody. Two glycosylated polypeptides (69-79K and 108-115K dalton) always co-precipitated and also appeared to induce serum neutralizing antibody.
Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/
Melissa Anne Lum
Lum, Melissa Anne, "Identification of bovine herpesvirus-1 polypeptides involved in serum neutralization " (1984). Retrospective Theses and Dissertations. 9005.