Degree Type

Thesis

Date of Award

2006

Degree Name

Master of Science

Department

Biochemistry, Biophysics and Molecular Biology

First Advisor

Basil J. Nikolau

Abstract

Biotin is an essential enzyme cofactor required for different metabolic processes. Plants are a major source of biotin, however, the genes and the protein products of biotin biosynthetic pathway are not fully characterized. We have molecularly characterized the genetically defined Arabidopsis bio1 locus as encoding 7,8-diaminopelargonic acid (DAPA) aminotransferase. Molecular and genetic analysis revealed that the full length BIO1 cDNA is capable of producing a fusion protein that catalyzes not only DAPA aminotransferase, but also dethiobiotin synthetase (BIO3) which is another enzymatic step in the biotin biosynthetic pathway. We refer to this fusion-gene as BIO3/BIO1. This bifunctional enzyme was demonstrated by functional complementation of E. coli bioA - and bioD- mutants deficient in DAPA aminotransferase and dethiobiotin synthetase reactions, respectively. This gene organization is unique in biology and establishes that the BIO3/BIO1 gene has dual functions.

DOI

https://doi.org/10.31274/rtd-180813-11169

Publisher

Digital Repository @ Iowa State University, http://lib.dr.iastate.edu

Copyright Owner

Elve Chen

Language

en

Proquest ID

AAI1440073

File Format

application/pdf

File Size

59 pages

Included in

Biochemistry Commons

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