Date of Award
Doctor of Philosophy
Cecil R. Stewart
We measured light and dark carbon dioxide exchange rates (CER) in germinating, presenescent, and senescing soybean cotyledons and in cotyledons rejuvenated by epicotyl removal on different days after planting. Soybean cotyledons achieved rates of net photosynthesis equivalent to rates that have been reported for true leaves. Light CER was greater than dark CER throughout most of the developmental sequence studied, indicating that soybean cotyledon photsynthesis provides for more than replacement of carbon respired in the dark. Rejuvenated cotyledons exhibited light CER equivalent to the maximal rates measured in presenescent cotyledons;In other aspects of cotyledon physiology, we observed significant differences between rejuvenated and presenescent soybean cotyledons. Rejuvenated cotyledons accumulated chlorophyll to levels greater than the maximal amount measured in presenescent cotyledons. A second difference was the observation that during rejuvenation, the Post-illumination outburst of CO[subscript]2 (the PIB), a qualitative indication of photorespiration, appeared to recover more slowly than light CER when compared with presenescent but equivalent light CERs;To investigate the relationship between the PIB and light CER, we labelled cotyledons with [superscript]14CO[subscript]2 during photosynthesis. Assuming that the percent of the total label incorporated that accumulated in glycine represents an independent indication of photorespiration, equivalent light CERs in rejuvenating and senescing cotyledons resulted in approximately equal photorespiration rates. Later in rejuvenation, however, the cotyledons appeared to have photorespiration rates greater than those seen in presenescent cotyledons;We measured the amount of ribulose-1,5-bisphosphate carboxylase oxygenase (RubisCO) in the cotyledons by rocket immunoelectrophoresis. Light CER was highly correlated with RubisCO concentration during most of the developmental sequence studied. Deviations from this relationship were seen during the earliest times of rejuvenation and in 4-day and 6-day old cotyledons. Light CER increased immediately after rejuvenation, without a change in RubisCO concentration. In 4-day and 6-day old cotyledons, light CER was much lower than expected based on the amount of RubisCO present.
Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/
Laura Fredrick Marek
Marek, Laura Fredrick, "Characterization of photorespiration and photosynthesis in soybean cotyledons during seedling development, cotyledon senescence and rejuvenation " (1988). Retrospective Theses and Dissertations. 9697.