Degree Type

Dissertation

Date of Award

1992

Degree Name

Doctor of Philosophy

Department

Veterinary Microbiology and Preventive Medicine

First Advisor

Merlin L. Kaeberle

Abstract

Immunization of young lambs against parainfluenza virus 3 (PI-3) is a major problem due to the presence of maternal antibodies that interfere with an active immune response. The objective of this experimentation was to develop a heterotypic vaccine that would overcome that blocking effect;Antiserums were produced in lambs against bovine PI-3 (BPI-3), human PI-3 (HPI-3), Sendai virus and simian virus-5 (SV-5). The titer and specificity of the antiserums were determined by hemagglutination inhibition (HI) test, enzyme linked immunosorbent assay (ELISA), Western immunoblot assay (WIA), and radioimmunoprecipitation assay (RIPA);These sheep antiserums demonstrated a close antigenic relationship between BPI-3 and HPI-3 viruses. Sendai virus was also found to share some epitopes with the PI-3 viruses. Antigenic crossreactivity of SV-5 antiserum was limited to the nucleoprotein of other viruses;Lambs with variant levels of PI-3 virus maternal antibodies were vaccinated with inactivated virus preparations of either bovine PI-3, human PI-3, or Sendai virus. The viruses were inactivated with binary ethylenimine, adjuvanted with aluminum hydroxide and administered in doses of 25 or 50 [mu]g (as protein) subcutaneously to groups of lambs. Primary immunization in the presence of maternal antibodies failed to induce a detectable response to bovine PI-3 virus. A marked antibody response developed to secondary immunization with BPI-3 virus vaccine in lambs with low maternal antibody titers (≤8). However, the secondary response was limited in lambs with high initial maternal antibody titers (≥16);Groups of lambs that had not been vaccinated or administered a single dose of Bovine PI-3 or Human PI-3 or two doses of Bovine PI-3 vaccine were challenged with an ovine strain of PI-3 virus. Clinical signs were most dramatic in the unvaccinated controls. The once-vaccinated Bovine PI-3 group also was markedly affected but the disease was much less severe in the once-vaccinated Human PI-3 and twice-vaccinated Bovine PI-3 groups. Results of this experimentation indicated some potential for the use of HPI-3 virus vaccine for control of PI-3 virus infection in lambs.

DOI

https://doi.org/10.31274/rtd-180813-12372

Publisher

Digital Repository @ Iowa State University, http://lib.dr.iastate.edu/

Copyright Owner

Manzoor Hussain

Language

en

Proquest ID

AAI9223933

File Format

application/pdf

File Size

178 pages

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