Recovery of Salmonella serotypes from swine faecal samples using ISO 6579

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1999
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van Winsen, R.
Swanenburg, M.
Snijders, J.
Urlings, H.
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International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork
Iowa State University Conferences and Symposia

The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.

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Standard procedures to exaruine the contamination of faecal saruples for salmonella are usually focussed towards the isolation of a single salmonella culture. Isolation procedures for salmonella consist of four distinct phases: I) non selective pre-enrichment; 2) selective enrichment; 3) selective isolation and elective growth to produce suspect isolates; 4) confmnation of the isolates. In most cases only one suspect colony is picked out for confmnation. In previous experiments we observed that more than one salmonella strain could be isolated if more than one suspect colony from the sarue saruple was investigated. Growth rates and sensitivity towards selective compounds during isolation procedures could influence the recovery of individual salmonella strains in a saruple. The objective of this study was to detennine whether the recovery rate of different salmonella strains/serotypes, present in one saruple, was equal for all serotypes.

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Fri Jan 01 00:00:00 UTC 1999