Estimation of sensitivity and specificity of an indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibodies against Salmonella enterica in meat juice and of microbiological examination of caecal content and mesenteric caecal lymph nodes for S. enterica
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The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.
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Abstract
The aim of this study was to estimate the sensitivity and specificity of an indirect enzyme-linked immunosorbent assay (ELISA) for detection ofantibodies against a variety of Salmonella enterica serovars in meat juice and of standard microbiological methods for detection of S. enterica in caecal content and in mesenteric caecal lymph nodes. Latent-class models were used for the estimation because no gold standard was available. The sensitivity of the ELISA was determined at different cut-off values of the optical density. The estimated sensitivity of the ELISA was 37%, 50% and 60% when the test was considered positive at an optical density ~ 40, 20 and 10, respectively. Estimates of the sensitivity of the microbiological examination of caecal content ranged from 32% to 39% and of the mesenteric caecal lymph nodes from 27% to 33%. The estimated specificity of all diagnostic procedures was 100%.