Comparison of two commercial ELISA for the diagnosis of salmonellosis in swine
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The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.
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Abstract
Three hundred and sixty-one pig sera collected in farms of Catalonia were randomly selected from a serum bank. Samples were examined by using two commercial ELISA kits. Results were compared with the kappa value using WinEpiscope. Besides, sample/positive ratios (S/P) were calculated. Comparison of results of both ELISA yielded a poor agreement (kappa 0.191), indicating that both ELISA did not measure the same. In addition, when raw optical densities were compared by means of a regression analysis, the results indicated a low correlation (r = 0.54 ). The results of this study clearly indicate that results of both kits are not interchangeable and that normalization of results by using S/P ratios did not serve to improve the agreement between tests. From our results, it is tempting to suggest that Salmotype detects a greater number of IgM positive pigs. The nature of these IgMs (salmonella-specific or not) is not known to us at this moment.