Development of a new molecular typing method of Salmonella spp. based on SNPs detection
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The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.
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Abstract
The aim of this study was to develop a new molecular typing method of Salmonella spp. based on the detection of point mutations (Single Nucleotide Polymorphisms or SNPs) in the genome. Several genomic regions (ManB, InvA, pduF) were sequenced in strains isolated from pig, the sequences were aligned and 49 point mutations were identified. A panel of 12 SNPs giving a genotype characteristic of a particular serotype was selected. Until now, thes selected SNPs were unable to distinguish the all strains. Therefore, additional SNPs must be selected.