Evaluation of the relative sensitivity of carcase swabbing against belly strip excision for TVC, E. coli and Salmonella isolation
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The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.
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Abstract
The standard US method of swabbmg p1g carcases (3x 100cm2) for determination of E. coli and Salmonella contamination was compared with a belly strip excision method (approx. 120cm2). Swabbing for Salmonella and E. coli detection was found to have a relative sensitivity equal to 1/ 7 and 1/ 2 respecl1vely, of the belly strip technique. Furthermore, swab sampling isolated 2 Salmonella serovars compared with 9 serovars by the belly strip technique. For studies on the effectiveness of carcass decontamination interventions or undertaking abattoir "flow-through" studies it is recommended that belly strip excision sampling be employed. This study also compared the use of a semisolid culture medium (MSRV) for Salmonella isolation developed for faecal samples with standard media. MSRV gave a result 24 hours faster but was not as sensitive as the standard medium (RV). Therefore, MSRV medium is not recommended for the isolation of Salmonella from carcases for regulatory purposes.