Event Title
Genetic characterization of Yersinia enterocolitica collected from tonsils of slaughtered pigs
Publication Date
January 2011
Abstract
From January to March 2009, detection of pathogenic Yersinia enterocolitica was done from 900 tonsils swabs collected from 45 pig batches in one slaughterhouse. 316 Y. enterocolitica isolates were collected and confirmed as pathogenic biotype by biochemical tests. For this study, these strains were genetically characterized on the basis of their virulence genes and their PFGE profiles. Real Time PCR was used to evaluate the presence of genes ail, myfA, and ystA on the genome and the gene yadA on the pYV plasmid. PFGE analysis using XbaI enzyme was also realised.
Book Title
21st International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork
Pages
53-56
Language
en
File Format
application/pdf
DOI
https://doi.org/10.31274/safepork-180809-573
Included in
Animal Diseases Commons, Animal Sciences Commons, Veterinary Microbiology and Immunobiology Commons, Veterinary Preventive Medicine, Epidemiology, and Public Health Commons
Genetic characterization of Yersinia enterocolitica collected from tonsils of slaughtered pigs
Maastricht, Netherlands
From January to March 2009, detection of pathogenic Yersinia enterocolitica was done from 900 tonsils swabs collected from 45 pig batches in one slaughterhouse. 316 Y. enterocolitica isolates were collected and confirmed as pathogenic biotype by biochemical tests. For this study, these strains were genetically characterized on the basis of their virulence genes and their PFGE profiles. Real Time PCR was used to evaluate the presence of genes ail, myfA, and ystA on the genome and the gene yadA on the pYV plasmid. PFGE analysis using XbaI enzyme was also realised.
