Publication Date

January 2011

Abstract

From January to March 2009, detection of pathogenic Yersinia enterocolitica was done from 900 tonsils swabs collected from 45 pig batches in one slaughterhouse. 316 Y. enterocolitica isolates were collected and confirmed as pathogenic biotype by biochemical tests. For this study, these strains were genetically characterized on the basis of their virulence genes and their PFGE profiles. Real Time PCR was used to evaluate the presence of genes ail, myfA, and ystA on the genome and the gene yadA on the pYV plasmid. PFGE analysis using XbaI enzyme was also realised.

Book Title

21st International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork

Pages

53-56

Language

en

File Format

application/pdf

DOI

https://doi.org/10.31274/safepork-180809-573

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Jan 1st, 12:00 AM

Genetic characterization of Yersinia enterocolitica collected from tonsils of slaughtered pigs

Maastricht, Netherlands

From January to March 2009, detection of pathogenic Yersinia enterocolitica was done from 900 tonsils swabs collected from 45 pig batches in one slaughterhouse. 316 Y. enterocolitica isolates were collected and confirmed as pathogenic biotype by biochemical tests. For this study, these strains were genetically characterized on the basis of their virulence genes and their PFGE profiles. Real Time PCR was used to evaluate the presence of genes ail, myfA, and ystA on the genome and the gene yadA on the pYV plasmid. PFGE analysis using XbaI enzyme was also realised.