Publication Date

January 2011

Abstract

A subset of swine farms in Ontario, Canada have been monitored for Cryptosporidium and Giardia. Fecal samples were collected from different stages of production as well as from manure pits. G. duodenalis cysts and Cryptosporidium spp. oocysts were detected in the manure samples using immunofluorescence microscopy. A nested PCR and sequencing method was performed to determine the genotypes. A mixed multivariable method was used to compare the prevalence of Cryptosporidium and Giardia in samples from different sources.

Book Title

25th International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork

Pages

70-73

Language

en

File Format

application/pdf

DOI

https://doi.org/10.31274/safepork-180809-577

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Jan 1st, 12:00 AM

Molecular epidemiology of Giardia duodenalis and Cryptosporidium spp on swine farms in Ontario, Canada

Maastricht, Netherlands

A subset of swine farms in Ontario, Canada have been monitored for Cryptosporidium and Giardia. Fecal samples were collected from different stages of production as well as from manure pits. G. duodenalis cysts and Cryptosporidium spp. oocysts were detected in the manure samples using immunofluorescence microscopy. A nested PCR and sequencing method was performed to determine the genotypes. A mixed multivariable method was used to compare the prevalence of Cryptosporidium and Giardia in samples from different sources.