Publication Date

January 2011

Abstract

A new selective chromogenic plate, YECA, was tested for its specificity, sensitivity and accuracy to detect pathogenic Y. enterocolitica from pig tonsils. We tested a panel of 26 bacterial strains on YECA and compared it to PCA, CIN and YeCM media. Detection of pathogenic Y. enterocolitica was carried out on 50 pig tonsils collected in one slaughterhouse. Enrichment was done in PSB and ITC broths. Streaking on YECA and CIN was done in direct, after 24H incubation of ITC, after 48H incubation of PSB and ITC. All the plates were incubated at 30°C during 24 hours. Presence of typical colonies on CIN and YECA was checked and isolates were biotyped.

Book Title

28th International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork

Pages

79-82

Language

en

File Format

application/pdf

DOI

https://doi.org/10.31274/safepork-180809-580

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Jan 1st, 12:00 AM

A selective chromogenic plate, YECA, for the detection of pathogenic Yersinia enterocolitica: specificity, sensibility and capacity to detect pathogenic Y. enterocolitica from pig tonsils

Maastricht, Netherlands

A new selective chromogenic plate, YECA, was tested for its specificity, sensitivity and accuracy to detect pathogenic Y. enterocolitica from pig tonsils. We tested a panel of 26 bacterial strains on YECA and compared it to PCA, CIN and YeCM media. Detection of pathogenic Y. enterocolitica was carried out on 50 pig tonsils collected in one slaughterhouse. Enrichment was done in PSB and ITC broths. Streaking on YECA and CIN was done in direct, after 24H incubation of ITC, after 48H incubation of PSB and ITC. All the plates were incubated at 30°C during 24 hours. Presence of typical colonies on CIN and YECA was checked and isolates were biotyped.