Publication Date

January 2011

Abstract

The quality of DNA extract significantly influences the outcome of PCR-based detection methods. Performances of four different DNA extraction methods were evaluated for their ability to recover Salmonella DNA from artificially contaminated specimens. Swine faecal and pork samples were spiked with known concentration of Salmonella Typhimurium, DNA was then extracted by each of the methods considered and finally tested by a commercial Salmonella Real Time kit.

Book Title

29th International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork

Pages

83-86

Language

en

File Format

application/pdf

DOI

https://doi.org/10.31274/safepork-180809-581

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Jan 1st, 12:00 AM

Comparison of DNA extraction methods to detect Salmonella spp. from pig faeces and pork

Maastricht, Netherlands

The quality of DNA extract significantly influences the outcome of PCR-based detection methods. Performances of four different DNA extraction methods were evaluated for their ability to recover Salmonella DNA from artificially contaminated specimens. Swine faecal and pork samples were spiked with known concentration of Salmonella Typhimurium, DNA was then extracted by each of the methods considered and finally tested by a commercial Salmonella Real Time kit.