EU regulations require disinfection of slaughter-equipment with water of at least 82ºC. However, according EU regulation 853/2004, an alternative system having an equivalent effect may be used when equivalence can be shown. Ecolab’s Inspexx© 200 (Inspexx) is a solution containing a stabilised blend of peracetic acid, peroctanoic acid and other organic acids – is shown to be an equivalent alternative. It is applied in water at ambient temperatures and does not need to be rinsed after application. A practical trial in a Dutch pork abattoir was performed to study whether Inspexx is adequately effective in disinfecting knives used at slaughter. The standard method of disinfection with water of 82°C is used as reference whereas Enterobacteriaceae and mesophilic aerobic counts are outcome variables. Knives are immersed in hot water or Inspexx for 0, 1, 10, 30 and 60 seconds respectively.