Publication Date

January 2011

Abstract

Food products of animal origin might play a role in interspecies transmission of C. difficile. In pigs, Clostridium difficile can cause neonatal enteritis and can be isolated from faeces from both diseased and healthy animals. To determine the prevalence of C. difficile in Dutch pigs arriving at the slaughterhouse a pilot study was conducted at one slaughterhouse in the Netherlands. Rectal faecal samples were taken from fifty slaughtering pigs from ten farms just after the pigs were sedated. These samples were examined using a real time PCR (BD GeneOhmTM Cdiff Assay), in combination with culturing after enrichment. Using real time PCR, none of the faecal samples were found to be positive for C. difficile while after culturing 14 samples (coming from pigs from nine different farms) were found to be positive for C. difficile. The positive samples derived from 9 different farms and encompassed seven different ribotypes.

Book Title

73rd International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork

Pages

214-216

Language

en

File Format

application/pdf

DOI

https://doi.org/10.31274/safepork-180809-625

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Jan 1st, 12:00 AM

Variety of Clostridium difficile PCR ribotypes in pigs arriving at the slaughterhouse

Maastricht, Netherlands

Food products of animal origin might play a role in interspecies transmission of C. difficile. In pigs, Clostridium difficile can cause neonatal enteritis and can be isolated from faeces from both diseased and healthy animals. To determine the prevalence of C. difficile in Dutch pigs arriving at the slaughterhouse a pilot study was conducted at one slaughterhouse in the Netherlands. Rectal faecal samples were taken from fifty slaughtering pigs from ten farms just after the pigs were sedated. These samples were examined using a real time PCR (BD GeneOhmTM Cdiff Assay), in combination with culturing after enrichment. Using real time PCR, none of the faecal samples were found to be positive for C. difficile while after culturing 14 samples (coming from pigs from nine different farms) were found to be positive for C. difficile. The positive samples derived from 9 different farms and encompassed seven different ribotypes.