Publication Date

January 2011

Abstract

ELISA represents a useful rapid method to detect the presence of specific antibodies on serum, plasma or meat juice collected at slaughter, however, false- and positive-results may occur depending on the sensitivity and specificity of the test. In this study we compare two commercial ELISA kits for the detection of specific antibodies against Trichinella spp. with respect to the gold standard method (artificial digestion) in a pig slaughterhouse. A total of 709 Iberian pigs belonging to 79 free-range herds were randomly selected and sampled (five to ten animals/herd) (Win Episcope 2.0; 95% confidence level, 8% accepted error).

Book Title

103rd International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork

Pages

310-312

Language

en

File Format

application/pdf

DOI

https://doi.org/10.31274/safepork-180809-654

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Jan 1st, 12:00 AM

Comparison of two commercial ELISA kits and magnetic stirrer method for detection of Trichinella spp. in a pig slaughterhouse

Maastricht, Netherlands

ELISA represents a useful rapid method to detect the presence of specific antibodies on serum, plasma or meat juice collected at slaughter, however, false- and positive-results may occur depending on the sensitivity and specificity of the test. In this study we compare two commercial ELISA kits for the detection of specific antibodies against Trichinella spp. with respect to the gold standard method (artificial digestion) in a pig slaughterhouse. A total of 709 Iberian pigs belonging to 79 free-range herds were randomly selected and sampled (five to ten animals/herd) (Win Episcope 2.0; 95% confidence level, 8% accepted error).