Publication Date
January 2011
Abstract
ELISA represents a useful rapid method to detect the presence of specific antibodies on serum, plasma or meat juice collected at slaughter, however, false- and positive-results may occur depending on the sensitivity and specificity of the test. In this study we compare two commercial ELISA kits for the detection of specific antibodies against Trichinella spp. with respect to the gold standard method (artificial digestion) in a pig slaughterhouse. A total of 709 Iberian pigs belonging to 79 free-range herds were randomly selected and sampled (five to ten animals/herd) (Win Episcope 2.0; 95% confidence level, 8% accepted error).
Book Title
103rd International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork
Pages
310-312
Language
en
File Format
application/pdf
DOI
https://doi.org/10.31274/safepork-180809-654
Included in
Animal Diseases Commons, Animal Sciences Commons, Veterinary Microbiology and Immunobiology Commons, Veterinary Preventive Medicine, Epidemiology, and Public Health Commons
Comparison of two commercial ELISA kits and magnetic stirrer method for detection of Trichinella spp. in a pig slaughterhouse
Maastricht, Netherlands
ELISA represents a useful rapid method to detect the presence of specific antibodies on serum, plasma or meat juice collected at slaughter, however, false- and positive-results may occur depending on the sensitivity and specificity of the test. In this study we compare two commercial ELISA kits for the detection of specific antibodies against Trichinella spp. with respect to the gold standard method (artificial digestion) in a pig slaughterhouse. A total of 709 Iberian pigs belonging to 79 free-range herds were randomly selected and sampled (five to ten animals/herd) (Win Episcope 2.0; 95% confidence level, 8% accepted error).
