Comparison of two commercial ELISA kits and magnetic stirrer method for detection of Trichinella spp. in a pig slaughterhouse
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The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.
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Abstract
ELISA represents a useful rapid method to detect the presence of specific antibodies on serum, plasma or meat juice collected at slaughter, however, false- and positive-results may occur depending on the sensitivity and specificity of the test. In this study we compare two commercial ELISA kits for the detection of specific antibodies against Trichinella spp. with respect to the gold standard method (artificial digestion) in a pig slaughterhouse. A total of 709 Iberian pigs belonging to 79 free-range herds were randomly selected and sampled (five to ten animals/herd) (Win Episcope 2.0; 95% confidence level, 8% accepted error).