Soybean Genetics Newsletter


Soybean isozymes have been studied genetically primarily with simple PAGE and starch gel electrophoresis (see Rennie et al., 1986, for a comparison and Kiang and Gorman, 1983, or Palmer et al., 1985, for reviews). Our objective was to test if the use of vertical gels with a PAGE concentration gradient would enhance isozyrne resolution over simple horizontal PAGE gels. Gradient gels were tested for their ability to resolve: amylase (Am), acid phosphatase (AP), Diaphorase (Dia), gl~cose-6-phosphate dehydrogenase (GPD), 6-phosphogluconate dehydrogenase (PGD), phosphoglucomutase (PGM) and superoxide dismutase/tetrazolium oxidase (SOD/TO) isozymes.