A procedure for aseptically culturing immature soybean cotyledons has been developed to study the synthesis of seed storage proteins. Experiments were carried out so that one cotyledon from an embryo was compared to the second cotyledon. Cotyledons were normally incubated for 6 days at 25 C in light with gentle shaking.
Thompson, John F. and Madison, James T.
"Research Notes: Cotyledon Culture,"
Soybean Genetics Newsletter: Vol. 3
, Article 28.
Available at: https://lib.dr.iastate.edu/soybeangenetics/vol3/iss1/28