Campus Units

Animal Science, Statistics

Document Type

Conference Proceeding

Conference

Animal Genomics for Animal Health

Publication Version

Published Version

Publication Date

2008

Journal or Book Title

Developments in Biologicals

Volume

132

First Page

105

Last Page

113

Conference Title

Animal Genomics for Animal Health

Conference Date

October 25-27, 2007

City

Paris, France

Abstract

We are investigating the porcine gut immune response to infection through gene expression profiling. Porcine Affymetrix GeneChip data was obtained from RNA prepared from mesenteric lymph node of swine infected with either Salmonella enterica serovar Typhimurium (ST) or S. Choleraesuis (SC) for 0, 8, 24, 48 or 504 hours post-inoculation (hpi). In total, 2,365 genes with statistical evidence for differential expression (DE; p < 0.01, q < 0.26, fold-change> 2) between at least two time-points were identified. Comparative Gene Ontology analyses revealed that a high proportion of annotated DE genes in both infections are involved in immune and defence responses. Hierarchical clustering of expression patterns and annotations showed that 22 of the 83 genes upregulated from 8-24 hpi in the SC infection are known NF-KB targets. The promoter sequences of human genes orthologous to the DE genes were collected and TFMExplorer was used to identify a set of 72 gene promoters with significant over-representation of NF-KB DNA-binding motifs. All 22 known NF-KB target genes are in this list; we hypothesize that the remaining 51 genes are un-recognized NF-KB targets. Integration of these results and verification of putative target genes will increase our understanding of the porcine response pathways responding to bacterial infection.

Comments

This proceeding is from Tuggle, C. K., Wang, Y. F., Couture, O. P., *Qu, L., Uthe, J. J., Kuhar, D., Lunney, J. K., Nettleton, D., Dekkers, J. C. M., Bearson, S. M. (2008). Computational integration of structural and functional genomics data across species to develop information on the porcine inflammatory gene regulatory pathway. In Pinard M-H, Gay C, Pastoret P-P, Dodet B (eds): Animal Genomics for Animal Health. Dev Biol (Basel). Basel, Karger, 2008, vol 132, pp 105-113.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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