Campus Units

Statistics

Document Type

Article

Publication Version

Published Version

Publication Date

2016

Journal or Book Title

Journal of Experimental Botany

Volume

67

Issue

4

First Page

1123

Last Page

1135

DOI

10.1093/jxb/erv513

Abstract

Maize develops a complex root system composed of embryonic and post-embryonic roots. Spatio-temporal differences in the formation of these root types imply specific functions during maize development. A comparative transcriptomic study of embryonic primary and seminal, and post-embryonic crown roots of the maize inbred line B73 by RNA sequencing along with anatomical studies were conducted early in development. Seminal roots displayed unique anatomical features, whereas the organization of primary and crown roots was similar. For instance, seminal roots displayed fewer cortical cell files and their stele contained more meta-xylem vessels. Global expression profiling revealed diverse patterns of gene activity across all root types and highlighted the unique transcriptome of seminal roots. While functions in cell remodeling and cell wall formation were prominent in primary and crown roots, stress-related genes and transcriptional regulators were over-represented in seminal roots, suggesting functional specialization of the different root types. Dynamic expression of lignin biosynthesis genes and histochemical staining suggested diversification of cell wall lignification among the three root types. Our findings highlight a cost-efficient anatomical structure and a unique expression profile of seminal roots of the maize inbred line B73 different from primary and crown roots.

Comments

This article is published as Tai, Huanhuan, Xin Lu, Nina Opitz, Caroline Marcon, Anja Paschold, Andrew Lithio, Dan Nettleton, and Frank Hochholdinger. "Transcriptomic and anatomical complexity of primary, seminal, and crown roots highlight root type-specific functional diversity in maize (Zea mays L.)." Journal of Experimental Botany 67, no. 4 (2015): 1123-1135. doi: 10.1093/jxb/erv513.

Creative Commons License

Creative Commons Attribution 3.0 License
This work is licensed under a Creative Commons Attribution 3.0 License.

Copyright Owner

The Authors

Language

en

File Format

application/pdf

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