Campus Units
Animal Science, Statistics, Veterinary Microbiology and Preventive Medicine
Document Type
Article
Publication Version
Published Version
Publication Date
12-2011
Journal or Book Title
PloS ONE
Volume
6
Issue
12
First Page
e28768
DOI
10.1371/journal.pone.0028768
Abstract
Transcriptomic analysis of the response to bacterial pathogens has been reported for several species, yet few studies have investigated the transcriptional differences in whole blood in subjects that differ in their disease response phenotypes. Salmonella species infect many vertebrate species, and pigs colonized with Salmonella enterica serovar Typhimurium (ST) are usually asymptomatic, making detection of these Salmonella-carrier pigs difficult. The variable fecal shedding of Salmonella is an important cause of foodborne illness and zoonotic disease. To investigate gene pathways and biomarkers associated with the variance in Salmonellashedding following experimental inoculation, we initiated the first analysis of the whole blood transcriptional response induced by Salmonella. A population of pigs (n = 40) was inoculated with ST and peripheral blood and fecal Salmonella counts were collected between 2 and 20 days post-inoculation (dpi). Two groups of pigs with either low shedding (LS) or persistent shedding (PS) phenotypes were identified. Global transcriptional changes in response to ST inoculation were identified by Affymetrix Genechip® analysis of peripheral blood RNA at day 0 and 2 dpi. ST inoculation triggered substantial gene expression changes in the pigs and there was differential expression of many genes between LS and PS pigs. Analysis of the differential profiles of gene expression within and between PS and LS phenotypic classes identified distinct regulatory pathways mediated by IFN-γ, TNF, NF-κB, or one of several miRNAs. We confirmed the activation of two regulatory factors, SPI1 and CEBPB, and demonstrated that expression of miR-155 was decreased specifically in the PS animals. These data provide insight into specific pathways associated with extremes in Salmonella fecal shedding that can be targeted for further exploration on why some animals develop a carrier state. This knowledge can also be used to develop rational manipulations of genetics, pharmaceuticals, nutrition or husbandry methods to decrease Salmonella colonization, shedding and spread.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Huang, Ting-Hua; Uthe, Jolita J.; Bearson, Shawn M. D.; Demirkale, Cumhur Yusuf; Nettleton, Dan; Knetter, Susan; Christian, Curtis; Ramer-Tait, Amanda E.; Wannemeuhler, Michael J.; and Tuggle, Christopher K., "Distinct Peripheral Blood RNA Responses to Salmonella in Pigs Differing in Salmonella Shedding Levels: Intersection of IFNG, TLR and miRNA Pathways" (2011). Statistics Publications. 202.
https://lib.dr.iastate.edu/stat_las_pubs/202
Included in
Computational Biology Commons, Large or Food Animal and Equine Medicine Commons, Microarrays Commons, Veterinary Preventive Medicine, Epidemiology, and Public Health Commons
Comments
This article is published as Huang T-H, Uthe JJ, Bearson SMD, Demirkale CY, Nettleton D, et al. (2011) Distinct Peripheral Blood RNA Responses to Salmonella in Pigs Differing in Salmonella Shedding Levels: Intersection of IFNG, TLR and miRNA Pathways. PLoS ONE 6(12): e28768. doi: 10.1371/journal.pone.0028768.