Campus Units

Animal Science, Statistics

Document Type

Article

Publication Version

Published Version

Publication Date

7-2007

Journal or Book Title

Genomics

Volume

91

Issue

1

First Page

72

Last Page

84

DOI

10.1016/j.ygeno.2007.03.018

Abstract

To elucidate the host transcriptional response to Salmonella enterica serovar Typhimurium, Affymetrix porcine GeneChip analysis of pig mesenteric lymph nodes was used to identify 848 genes showing differential expression across different times after inoculation or when compared to non-inoculated controls. Annotation analyses showed that a high proportion of these differentially expressed (DE) genes are involved in immune and inflammatory responses. T helper 1, innate/inflammatory, and antigen-processing pathways were induced at 24 h post-inoculation (hpi) and/or 48 hpi, while apoptosis and antigen presentation/dendritic cell function pathways were downregulated at 8 hpi. Cluster analyses revealed that most DE genes annotated as NFκB targets were grouped into a specific induced subcluster, while many translation-related DE genes were found in a repressed subcluster. Quantitative polymerase chain reaction analyses confirmed the Affymetrix results, revealing transcriptional induction of NFκB target genes at 24 hpi and suppression of the NFκB pathway from 24 to 48 hpi. We propose that such NFκB suppression in antigen-presenting cells may be the mechanism by which S. Typhimurium eludes a strong inflammatory response to establish a carrier status in pigs.

Comments

This article is published as Wang, Yanfang, Long Qu, Jolita J. Uthe, Shawn MD Bearson, Daniel Kuhar, Joan K. Lunney, Oliver P. Couture, Dan Nettleton, Jack CM Dekkers, and Christopher K. Tuggle. "Global transcriptional response of porcine mesenteric lymph nodes to Salmonella enterica serovar Typhimurium." Genomics 90, no. 1 (2007): 72-84. doi: 10.1016/j.ygeno.2007.03.018.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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