Campus Units
Agronomy, Statistics
Document Type
Article
Publication Version
Published Version
Publication Date
5-2002
Journal or Book Title
Plant Physiology
Volume
129
Issue
1
First Page
13
Last Page
22
DOI
10.1104/pp.000653
Abstract
We have achieved routine transformation of maize (Zea mays) using an Agrobacterium tumefaciensstandard binary (non-super binary) vector system. Immature zygotic embryos of the hybrid line Hi II were infected with A. tumefaciens strain EHA101 harboring a standard binary vector and cocultivated in the presence of 400 mg L−1 l-cysteine. Inclusion of l-cysteine in cocultivation medium lead to an improvement in transient β-glucuronidase expression observed in targeted cells and a significant increase in stable transformation efficiency, but was associated with a decrease in embryo response after cocultivation. The average stable transformation efficiency (no. of bialaphos-resistant events recovered per 100 embryos infected) of the present protocol was 5.5%. Southern-blot and progeny analyses confirmed the integration, expression, and inheritance of the bar andgustransgenes in R0, R1, and R2 generations of transgenic events. To our knowledge, this represents the first report in which fertile, stable transgenic maize has been routinely produced using an A. tumefaciensstandard binary vector system.
Copyright Owner
American Society of Plant Physiologists
Copyright Date
2002
Language
en
File Format
application/pdf
Recommended Citation
Frame, Bronwyn R.; Shou, Huixia; Chikwamba, Rachel K.; Zhang, Zhanyuan; Xiang, Chengbin; Fonger, Tina M.; Pegg, Sue Ellen K.; Li, Baochun; Nettleton, Dan; Pei, Deqing; and Wang, Kan, "Agrobacterium tumefaciens-Mediated Transformation of Maize Embryos Using a Standard Binary Vector System" (2002). Statistics Publications. 226.
https://lib.dr.iastate.edu/stat_las_pubs/226
Included in
Agronomy and Crop Sciences Commons, Applied Statistics Commons, Plant Breeding and Genetics Commons, Plant Pathology Commons, Statistical Methodology Commons
Comments
This article is published as Frame, Bronwyn R., Huixia Shou, Rachel K. Chikwamba, Zhanyuan Zhang, Chengbin Xiang, Tina M. Fonger, Sue Ellen K. Pegg et al. "Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system." Plant physiology 129, no. 1 (2002): 13-22. doi: 10.1104/pp.000653. Posted with permission.