Campus Units

Agronomy, Statistics

Document Type

Article

Publication Version

Published Version

Publication Date

5-2002

Journal or Book Title

Plant Physiology

Volume

129

Issue

1

First Page

13

Last Page

22

DOI

10.1104/pp.000653

Abstract

We have achieved routine transformation of maize (Zea mays) using an Agrobacterium tumefaciensstandard binary (non-super binary) vector system. Immature zygotic embryos of the hybrid line Hi II were infected with A. tumefaciens strain EHA101 harboring a standard binary vector and cocultivated in the presence of 400 mg L−1 l-cysteine. Inclusion of l-cysteine in cocultivation medium lead to an improvement in transient β-glucuronidase expression observed in targeted cells and a significant increase in stable transformation efficiency, but was associated with a decrease in embryo response after cocultivation. The average stable transformation efficiency (no. of bialaphos-resistant events recovered per 100 embryos infected) of the present protocol was 5.5%. Southern-blot and progeny analyses confirmed the integration, expression, and inheritance of the bar andgustransgenes in R0, R1, and R2 generations of transgenic events. To our knowledge, this represents the first report in which fertile, stable transgenic maize has been routinely produced using an A. tumefaciensstandard binary vector system.

Comments

This article is published as Frame, Bronwyn R., Huixia Shou, Rachel K. Chikwamba, Zhanyuan Zhang, Chengbin Xiang, Tina M. Fonger, Sue Ellen K. Pegg et al. "Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system." Plant physiology 129, no. 1 (2002): 13-22. doi: 10.1104/pp.000653. Posted with permission.

Copyright Owner

American Society of Plant Physiologists

Language

en

File Format

application/pdf

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