Campus Units
Plant Pathology and Microbiology, Statistics
Document Type
Article
Publication Version
Published Version
Publication Date
4-2007
Journal or Book Title
Molecular Plant-Microbe Interactions
Volume
20
Issue
4
First Page
358
Last Page
370
DOI
10.1094/MPMI-20-4-0358
Abstract
Virus-infected leaf tissues comprise a heterogeneous mixture of cells at different stages of infection. The spatial and temporal relationships between sites of virus accumulation and the accompanying host responses, such as altered host gene expression, are not well defined. To address this issue, we utilized Turnip mosaic virus (TuMV) tagged with the green fluorescent protein to guide the dissection of infection foci into four distinct zones. The abundance of Arabidopsis thaliana mRNA transcripts in each of the four zones then was assayed using the Arabidopsis ATH1 GeneChip oligonucleotide microarray (Affymetrix). mRNA transcripts with significantly altered expression profiles were determined across gradients of virus accumulation spanning groups of cells in and around foci at different stages of infection. The extent to which TuMV-responsive genes were up- or downregulated primarily correlated with the amount of virus accumulation regardless of gene function. The spatial analysis also allowed new suites of coordinately regulated genes to be identified that are associated with chloroplast functions (decreased), sulfate assimilation (decreased), cell wall extensibility (decreased), and protein synthesis and turnover (induced). The functions of these downregulated genes are consistent with viral symptoms, such as chlorosis and stunted growth, providing new insight into mechanisms of pathogenesis.
Copyright Owner
The American Phytopathological Society
Copyright Date
2007
Language
en
File Format
application/pdf
Recommended Citation
Yang, Chunling; Guo, Rong; Jie, Fei; Nettleton, Dan; Peng, Jiqing; Carr, Tyrell; Yeakley, Joanne M.; Fan, Jian-Bing; and Whitham, Steven A., "Spatial Analysis of Arabidopsis thaliana Gene Expression in Response to Turnip mosaic virus Infection" (2007). Statistics Publications. 231.
https://lib.dr.iastate.edu/stat_las_pubs/231
Included in
Genetics and Genomics Commons, Microarrays Commons, Plant Biology Commons, Plant Pathology Commons
Comments
This article is published as Yang, Chunling, Rong Guo, Fei Jie, Dan Nettleton, Jiqing Peng, Tyrell Carr, Joanne M. Yeakley, Jian-Bing Fan, and Steven A. Whitham. "Spatial analysis of Arabidopsis thaliana gene expression in response to Turnip mosaic virus infection." Molecular plant-microbe interactions 20, no. 4 (2007): 358-370. doi: 10.1094/MPMI-20-4-0358. Posted with permission.