Transcript-Based Cloning of RRP46, a Regulator of rRNA Processing and R Gene–Independent Cell Death in Barley–Powdery Mildew Interactions

Xi, Liu; Nettleton, Dan; Moscou, Matthew; Meng, Yan; Xu, Weihui; Caldo, Rico; Shaver, Miranda; Nettleton, Dan; Wise, Roger

Transcript-Based Cloning of RRP46, a Regulator of rRNA Processing and R Gene–Independent Cell Death in Barley–Powdery Mildew Interactions

File

2009_Nettleton_TranscriptBased.pdf (2.12 MB)

Date

2009-10-01

Authors

Xi, Liu

Nettleton, Dan

Moscou, Matthew

Meng, Yan

Xu, Weihui

Caldo, Rico

Shaver, Miranda

Nettleton, Dan

Wise, Roger

Authors

Person

Nettleton, Dan

Department Chair and Distinguished Professor

Organizational Units

Organizational Unit

Plant Pathology and Microbiology

The Department of Plant Pathology and Microbiology and the Department of Entomology officially merged as of September 1, 2022. The new department is known as the Department of Plant Pathology, Entomology, and Microbiology (PPEM). The overall mission of the Department is to benefit society through research, teaching, and extension activities that improve pest management and prevent disease. Collectively, the Department consists of about 100 faculty, staff, and students who are engaged in research, teaching, and extension activities that are central to the mission of the College of Agriculture and Life Sciences. The Department possesses state-of-the-art research and teaching facilities in the Advanced Research and Teaching Building and in Science II. In addition, research and extension activities are performed off-campus at the Field Extension Education Laboratory, the Horticulture Station, the Agriculture Engineering/Agronomy Farm, and several Research and Demonstration Farms located around the state. Furthermore, the Department houses the Plant and Insect Diagnostic Clinic, the Iowa Soybean Research Center, the Insect Zoo, and BugGuide. Several USDA-ARS scientists are also affiliated with the Department.

Organizational Unit

Statistics

As leaders in statistical research, collaboration, and education, the Department of Statistics at Iowa State University offers students an education like no other. We are committed to our mission of developing and applying statistical methods, and proud of our award-winning students and faculty.

Organizational Unit

Bioinformatics and Computational Biology

Department

Plant Pathology and MicrobiologyStatisticsBioinformatics and Computational Biology

Abstract

Programmed cell death (PCD) plays a pivotal role in plant development and defense. To investigate the interaction between PCD and R gene–mediated defense, we used the 22K Barley1 GeneChip to compare and contrast time-course expression profiles of Blumeria graminis f. sp hordei (Bgh) challenged barley (Hordeum vulgare) cultivar C.I. 16151 (harboring the Mla6 powdery mildew resistance allele) and its fast neutron–derived Bgh-induced tip cell death1 mutant, bcd1. Mixed linear model analysis identified genes associated with the cell death phenotype as opposed to Rgene–mediated resistance. One-hundred fifty genes were found at the threshold P value < 0.0001 and a false discovery rate bcd1mutant. Gene Ontology and rice (Oryza sativa) alignment-based annotation indicated that 68 of the 124 overexpressed genes encode ribosomal proteins. A deletion harboring six genes on chromosome 5H cosegregates with bcd1-specified cell death and is associated with misprocessing of rRNAs but segregates independent of R gene–mediated resistance. Barley stripe mosaic virus-induced gene silencing of one of the six deleted genes, RRP46 (rRNA-processing protein 46), phenocopied bcd1-mediated tip cell death. These findings suggest that RRP46, a critical component of the exosome core, mediates RNA processing and degradation involved in cell death initiation as a result of attempted penetration by Bgh during the barley–powdery mildew interaction but is independent of gene-for-gene resistance.

Comments

This article is published as Xi, Liu, Matthew J. Moscou, Yan Meng, Weihui Xu, Rico A. Caldo, Miranda Shaver, Dan Nettleton, and Roger P. Wise. "Transcript-based cloning of RRP46, a regulator of rRNA processing and R gene–independent cell death in barley–powdery mildew interactions." The Plant Cell 21, no. 10 (2009): 3280-3295. doi: 10.1105/tpc.109.066167.