Campus Units

Veterinary Diagnostic and Production Animal Medicine

Document Type

Article

Publication Version

Published Version

Publication Date

2015

Journal or Book Title

Journal of Swine Health and Production

Volume

23

Issue

2

First Page

84

Last Page

90

Abstract

Objectives: To determine temperature and time applications sufficient to inactivate porcine epidemic diarrhea virus (PEDV) on a commercial livestock trailer, and practical within the constraints of current thermo-assisted drying and decontamination (TADD) capabilities in the industry.

Materials and methods: Thirty-two 4-week-old barrows were inoculated via oral gastric tube with 5 mL of either PEDV-negative feces (Neg; n = 4), untreated PEDV-positive feces (Pos; n = 4), or PEDV-positive feces subjected to 71°C for 10 minutes (71C-10M; n = 4), 63°C for 10 minutes (63C-10M; n = 4), 54°C for 10 minutes (54C-10M; n = 4), 38°C for 12 hours (38C-12H; n = 4), 20°C for 24 hours (20C-24H; n = 4), or 20°C for 7 days (20C-7D; n = 4). These pigs served as a bioassay to determine the infectivity of virus following treatment. Bioassay results were determined by reverse-transcriptase polymerase chain reaction on rectal swabs collected from the inoculated pigs on days 3 and 7 post inoculation.

Results: None of the pigs in the 71C-10M and 20C-7D groups became infected with PEDV. This result differed significantly from that of the Pos group (P < .05). Results of the other groups did not differ significantly from that of the Pos group (P > .05).

Implication: Holding PEDV in the presence of feces at 71°C for 10 minutes or at 20°C (room temperature) for 7 days is sufficient to inactivate the virus, preventing transmission under the conditions of this study.

Comments

This article is published as Thomas, Paul R., Locke A. Karriker, Alejandro Ramirez, Jianqiang Zhang, Josh S. Ellingson, Kimberly K. Crawford, Jessica L. Bates, Kristin J. Hammen, and Derald J. Holtkamp. "Evaluation of time and temperature sufficient to inactivate porcine epidemic diarrhea virus in swine feces on metal surfaces." Journal of Swine Health and Production 23, no. 2 (2015): 84-90.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en, es, fr

File Format

application/pdf

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