Title
Ethyl pyruvate reduces organic dust-induced airway inflammation by targeting HMGB1-RAGE signaling
Campus Units
Biomedical Sciences, Veterinary Diagnostic and Production Animal Medicine
Document Type
Article
Publication Version
Published Version
Publication Date
12-2019
Journal or Book Title
Respiratory Research
Volume
20
Issue
1
First Page
27
DOI
10.1186/s12931-019-0992-3
Abstract
Background
Animal production workers are persistently exposed to organic dust and can suffer from a variety of respiratory disease symptoms and annual decline in lung function. The role of high mobility group box-1 (HMGB1) in inflammatory airway diseases is emerging. Hence, we tested a hypothesis that organic dust exposure of airway epithelial cells induces nucleocytoplasmic translocation of HMGB1 and blocking this translocation dampens organic dust-induced lung inflammation.
Methods
Rats were exposed to either ambient air or swine barn (8 h/day for either 1, 5, or 20 days) and lung tissues were processed for immunohistochemistry. Swine barn dust was collected and organic dust extract (ODE) was prepared and sterilized. Human airway epithelial cell line (BEAS-2B) was exposed to either media or organic dust extract followed by treatment with media or ethyl pyruvate (EP) or anti-HMGB1 antibody. Immunoblotting, ELISA and other assays were performed at 0 (control), 6, 24 and 48 h. Data (as mean ± SEM) was analyzed using one or two-way ANOVA followed by Bonferroni’s post hoc comparison test. A p value of less than 0.05 was considered significant.
Results
Compared to controls, barn exposed rats showed an increase in the expression of HMGB1 in the lungs. Compared to controls, ODE exposed BEAS-2B cells showed nucleocytoplasmic translocation of HMGB1, co-localization of HMGB1 and RAGE, reactive species and pro-inflammatory cytokine production. EP treatment reduced the ODE induced nucleocytoplasmic translocation of HMGB1, HMGB1 expression in the cytoplasmic fraction, GM-CSF and IL-1β production and augmented the production of TGF-β1 and IL-10. Anti-HMGB1 treatment reduced ODE-induced NF-κB p65 expression, IL-6, ROS and RNS but augmented TGF-β1 and IL-10 levels.
Conclusions
HMGB1-RAGE signaling is an attractive target to abrogate OD-induced lung inflammation.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Copyright Owner
The Authors
Copyright Date
2019
Language
en
File Format
application/pdf
Recommended Citation
Bhat, Sanjana Mahadev; Massey, Nyzil; Karriker, Locke A.; Singh, Baljit; and Charavaryamath, Chandrashekhar, "Ethyl pyruvate reduces organic dust-induced airway inflammation by targeting HMGB1-RAGE signaling" (2019). Veterinary Diagnostic and Production Animal Medicine Publications. 145.
https://lib.dr.iastate.edu/vdpam_pubs/145
Comments
This article is published as Bhat, Sanjana Mahadev, Nyzil Massey, Locke A. Karriker, Baljit Singh, and Chandrashekhar Charavaryamath. "Ethyl pyruvate reduces organic dust-induced airway inflammation by targeting HMGB1-RAGE signaling." Respiratory Research 20, no. 1 (2019): 27. DOI: 10.1186/s12931-019-0992-3. Posted with permission.