Campus Units
Veterinary Microbiology and Preventive Medicine
Document Type
Article
Publication Version
Published Version
Publication Date
12-10-2019
Journal or Book Title
Microorganisms
Volume
7
Issue
12
First Page
675
DOI
10.3390/microorganisms7120675
Abstract
We investigated the anti-Campylobacter activity of pinocembrin and its mechanism of action, as well as Campylobacter responses to pinocembrin treatment at the genetic and phenotypic levels, using C. jejuni NCTC 11168 and a multidrug efflux system repressor mutant (11168ΔcmeR). At its minimal inhibitory concentration, pinocembrin significantly increased cell membrane permeability of Campylobacter. Interestingly, at sub-inhibitory concentrations, pinocembrin did not significantly alter membrane functionality and it increased bacterial fitness. Treatment with pinocembrin evoked decreased expression of ribosomal proteins and down-regulation of several NADH dehydrogenase I chain subunits and proteins involved in iron uptake. This suggests altered protein production and redox cycle and iron metabolism. Interestingly, the chelation of Fe ions during the treatment with pinocembrin increased C. jejuni survival, although there was no increase in the formation of reactive oxygen species. Pre-treatment of C. jejuni with sub-inhibitory concentrations of pinocembrin for 2 h resulted in a 1 log decrease in C. jejuni colony forming units in mice liver at 8 days post-infection, compared to untreated C. jejuni. These findings suggest that pinocembrin modulates the metabolic activity of C. jejuni and that pre-treatment of C. jejuni with pinocembrin influences its virulence potential in mice. This anti-Campylobacter potential of pinocembrin warrants further investigation.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Copyright Owner
The Authors
Copyright Date
2019
Language
en
File Format
application/pdf
Recommended Citation
Klančnik, Anja; Šimunović, Katarina; Kovac, Jasna; Sahin, Orhan; Wu, Zuowei; Vučković, Darinka; Abram, Maja; Zhang, Qijing; and Možina, Sonja Smole, "The Anti-Campylobacter Activity and Mechanisms of Pinocembrin Action" (2019). Veterinary Diagnostic and Production Animal Medicine Publications. 165.
https://lib.dr.iastate.edu/vdpam_pubs/165
Included in
Food Microbiology Commons, Veterinary Microbiology and Immunobiology Commons, Veterinary Toxicology and Pharmacology Commons
Comments
This article is published as Klančnik, Anja, Katarina Šimunović, Jasna Kovac, Orhan Sahin, Zuowei Wu, Darinka Vučković, Maja Abram, Qijing Zhang, and Sonja Smole Možina. "The Anti-Campylobacter Activity and Mechanisms of Pinocembrin Action." Microorganisms 7, no. 12 (2019): 675. DOI: 10.3390/microorganisms7120675. Posted with permission.