Title
Practical aspects of PRRSV RNA detection in processing fluids collected in commercial swine farms
Campus Units
Veterinary Diagnostic and Production Animal Medicine, Statistics, Veterinary Diagnostic Laboratory
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
7-2020
Journal or Book Title
Preventive Veterinary Medicine
Volume
180
First Page
105021
DOI
10.1016/j.prevetmed.2020.105021
Abstract
Processing fluid samples are easily collected under field conditions and provide the means to test more piglets more frequently in a practical way, thereby improving PRRSV surveillance. However, a deeper understanding of the diagnostic characteristics of this newly described sample type is still required. Therefore, the objective of this field-based study was to determine the relationship between viremic piglets and the detection of PRRSV RNA in processing fluid samples. In two PRRSV-positive breeding herds, processing fluids (n = 77) and individual piglet serum samples (n = 834) were collected from 77 litters in three sampling events and tested for PRRSV RNA. Among the 77 litters in the study, 55 litters (71.4%) contained no viremic piglets and processing fluids tested negative for PRRSV RNA. Among the 22 (28.6%) litters with ≥1 viremic piglets, 10 litters contained a single viremic piglet and 5 of the 10 processing fluids from this group tested positive for PRRSV RNA. Based on a fitted mixed effects logistic regression model, the probability of detecting PRRSV RNA in processing fluids was highly dependent on the number of viremic piglets contributing to the sample. When the within-litter prevalence was ≥39%, the probability of detecting PRRSV RNA in processing fluids was ≥95%. By extension, the results suggest that pooling processing fluids from several litters increases the probability of PRRSV RNA detection because of the greater likelihood of including multiple litters each with ≥1 viremic piglets. In contemporary breeding herds that use processing fluid samples for PRRSV surveillance, the diagnostic costs associated with testing 100% of the processing-age piglet population can be estimated at €0.077 ($0.086 USD) per pig weaned. In contrast, to achieve an equivalent testing coverage with the use of individual piglet serum samples, the diagnostic costs associated would be €4.48 ($5.00 USD) per pig weaned. Processing fluid represents a practical, reliable and efficient method to surveil breeding herds for PRRSV because it allows for continuous surveillance at a low cost.
Copyright Owner
Elsevier B.V.
Copyright Date
2020
Language
en
File Format
application/pdf
Recommended Citation
López, Will A.; Zimmerman, Jeffrey J.; Gauger, Phillip C.; Harmon, Karen M.; Bradner, Laura K.; Zhang, Min; Giménez-Lirola, Luis; Ramirez, Alejandro; Cano, Jean Paul; and Linhares, Daniel C. L., "Practical aspects of PRRSV RNA detection in processing fluids collected in commercial swine farms" (2020). Veterinary Diagnostic and Production Animal Medicine Publications. 190.
https://lib.dr.iastate.edu/vdpam_pubs/190
Included in
Large or Food Animal and Equine Medicine Commons, Veterinary Infectious Diseases Commons, Veterinary Preventive Medicine, Epidemiology, and Public Health Commons
Comments
This is a manuscript of an article published as López, Will, Jeffrey J. Zimmerman, Phil Gauger, Karen Harmon, Laura Bradner, Min Zhang, Luis Giménez-Lirola, Alejandro Ramirez, Jean Paul Cano, and Daniel CL Linhares. "Practical aspects of PRRSV RNA detection in processing fluids collected in commercial swine farms." Preventive Veterinary Medicine (2020): 105021. DOI: 10.1016/j.prevetmed.2020.105021. Posted with permission.