Campus Units
Veterinary Diagnostic and Production Animal Medicine, Statistics
Document Type
Article
Publication Version
Published Version
Publication Date
12-2014
Journal or Book Title
International Journal of Veterinary Science and Medicine
Volume
2
Issue
2
First Page
109
Last Page
113
DOI
10.1016/j.ijvsm.2014.08.001
Abstract
Porcine proliferative enteropathy (PPE) is a common and economically important gastro-intestinal disease of swine caused by the intracellular bacterium, Lawsonia intracellularis. Conventional tests to detect antibody responses to L. intracellularisinclude the immuno-peroxidase monolayer assay (IPMA), immuno-fluorescent antibody test (IFAT) and a lipopolysaccharide ELISA (LPS-ELISA). These tests are not commercially available. Therefore, objective of this study is to evaluate the performance of a commercial L. intracellularis blocking ELISA. Performance of the commercial ELISA was compared to the IPMA and LPS-ELISA using serum from experimentally infected animals (N = 40). The prevalence of L. intracellularis sero-positive animals was assessed by comparing suspect and randomly selected sera (N = 394). The commercial ELISA, IPMA and a non-commercial lipopolysaccharide (LPS) LPS-ELISA showed a 95% correlation when tested using experimentally derived known status samples. When compared to the IPMA the sensitivity of the commercial ELISA was 91% while the specificity was 100%. Therefore, the diagnostic sensitivity and specificity of the commercial L. intracellularis ELISA was comparable to the LPS-ELISA and IPMA. A comparison of suspect and randomly selected field samples with the commercial ELISA indicated that L. intracellularis sero-positivity is widespread and does not correlate with possible disease status.
Rights
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/ licenses/by-nc-nd/3.0/).
Copyright Owner
Elsevier
Copyright Date
2014
Language
en
File Format
application/pdf
Recommended Citation
Magtoto, Ronaldo L.; Vegi, Anuradha; Wang, Chong; Johnson, John K.; and Ramamoorthy, Sheela, "Evaluation and use of a serological assay for the detection of antibodies to Lawsonia intracellularis in swine" (2014). Veterinary Diagnostic and Production Animal Medicine Publications. 59.
https://lib.dr.iastate.edu/vdpam_pubs/59
Included in
Large or Food Animal and Equine Medicine Commons, Statistical Methodology Commons, Veterinary Microbiology and Immunobiology Commons, Veterinary Physiology Commons
Comments
This article is from International Journal of Veterinary Science and Medicine 2 (2014); 109, doi: 10.1016/j.ijvsm.2014.08.001. Posted with permission.