Campus Units

Veterinary Microbiology and Preventive Medicine

Document Type

Article

Publication Version

Published Version

Publication Date

10-2007

Journal or Book Title

Journal of Becteriology

Volume

189

Issue

20

First Page

7399

Last Page

7407

DOI

10.1128/JB.01012-07

Abstract

Campylobacter jejuni is an important bacterial pathogen causing gastroenteritis in humans. C. jejuni is capable of natural transformation, which is considered a major mechanism mediating horizontal gene transfer and generating genetic diversity. Despite recent efforts to elucidate the transformation mechanisms of C. jejuni, the process of DNA binding and uptake in this organism is still not well understood. In this study, we report a previously unrecognized DNA-binding protein (Cj0011c) in C. jejuni that contributes to natural transformation. Cj0011c is a small protein (79 amino acids) with a partial sequence homology to the C-terminal region of ComEA in Bacillus subtilis. Cj0011c bound to both single- and double-stranded DNA. The DNA-binding activity of Cj0011c was demonstrated with a variety of DNAs prepared from C. jejuni or Escherichia coli, suggesting that the DNA binding of Cj0011c is not sequence dependent. Deletion of the cj0011c gene from C. jejuni resulted in 10- to 50-fold reductions in the natural transformation frequency. Different from the B. subtilis ComEA, which is an integral membrane protein, Cj0011c is localized in the periplasmic space of C. jejuni. These results indicate that Cj0011c functions as a periplasmic DNA receptor contributing to the natural transformation of C. jejuni.

Comments

This article is published as Jeon, Byeonghwa, and Qijing Zhang. "Cj0011c, a periplasmic single-and double-stranded DNA-binding protein, contributes to natural transformation in Campylobacter jejuni." Journal of bacteriology 189, no. 20 (2007): 7399-7407.doi: 10.1128/JB.01012-07. Posted with permission.

Copyright Owner

American Society for Microbiology

Language

en

File Format

application/pdf

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